Pyridoxylated Hemoglobin Solution as an Oxygen Carrier
نویسندگان
چکیده
منابع مشابه
Use of Liposome Encapsulated Hemoglobin (leh) as an Oxygen Carrier to Cultured Cells
Oxygen transport is the most constraining parameter to engineer three-dimensional (3 D) tissues in vitro. The concentration of oxygen dissolved in the culture medium according to the Henry's law is very low (about 2×10 mol/mL under 21%-O2 containing air), and it is not likely to satisfy the metabolic demand of a high cell density tissue such as liver (e.g., 2.5 ×10 parenchymal cells/mL-in vivo ...
متن کاملPharmacokinetic study of enclosed hemoglobin and outer lipid component after the administration of hemoglobin vesicles as an artificial oxygen carrier.
The hemoglobin vesicle (HbV) is an artificial oxygen carrier that encapsulates a concentrated Hb solution in lipid vesicles (liposomes). The pharmacokinetic properties of HbV were investigated in mice and rats. With use of HbV in which the internal Hb was labeled with (125)I ((125)I-HbV) and cell-free (125)I-Hb, it was found that encapsulation of Hb increased the half-life by 30 times, accompan...
متن کاملReexamination of the polymerization of pyridoxylated hemoglobin with glutaraldehyde.
Pyridoxylated adult human hemoglobin (HbAo) was prepared using a one molar equivalent of pyridoxal 5-phosphate (PLP) per heme and reduced with either NaCNBH3 or NaBH4. A separate sample was pyridoxylated and passed through a mixed-bed ion exchange column without reduction. All three preparations had a P50 of 29 +/- 2 torr and a cooperativity of n = 2.4 +/- 0.1. These preparations, in both the o...
متن کاملMicrocirculation and NO-CO studies of a natural extracellular hemoglobin developed for an oxygen therapeutic carrier.
Extracellular soluble hemoglobins (Hbs) have long been studied for their possible use as safe and effective alternatives to blood transfusion. While remarkable progress has been made in the use of cell-free Hb as artificial oxygen carrier, significant problems remain, including susceptibility to oxidative inactivation and propensity to induce vasoconstriction. Hemarina-M101 is a natural giant e...
متن کاملNumerical study of oxygen uptake by layers of hemoglobin solution.
Numerical solutions have been obtained for the equations describing 02 uptake by layers of concentrated hemoglobin solution 0.25, 0.5, 1, 1.6, 2, 3.6, 5, 10, and 20 p thick. The results indicate that the diffusion of oxyhemoglobin has almost no effect on the rate of oxygenation of the 0.25 p layer, but its influence increases with increasing layer thickness, so that it shortens the time require...
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ژورنال
عنوان ژورنال: Annals of Surgery
سال: 1990
ISSN: 0003-4932
DOI: 10.1097/00000658-199004000-00001